This system is based on a co-expression mechanism using custom plasmids, Taking the novel coronavirus as an example, it combines recombinant green fluorescent protein (rGFP) with the coronavirus 3CL protease. In this system, the rGFP sequence incorporates specific cleavage sites for the 3CL protease.

The DIFF-HPV pseudovirus developed by Difu Runs Biotech encompasses multiple reporter genes such as GFP, RFP, BFP, and Luciferase. It features high signal-to-noise ratio, strong stability (no effect on pseudovirus after five repeated freeze-thaw cycles), and only requires culture in ordinary 293T cells. It includes the main types such as HPV16, 18, 6, 11, 31, 33, 45 and 52, which are nine-valent. Pseudoviruses themselves have the characteristic of single-round infection and have low requirements for laboratory biosafety levels, making them highly suitable for the HPV neutralization assay PBNA method (pseudovirus based neutralization assay, PBNA).

The viruses of our company originate from the world's largest repository of pathogenic microorganisms and the National Microbial and Virus Strain Preservation Center, with a comprehensive range of virus types and subtypes. The viral strains are authenticated by authoritative microbial strain and virus strain preservation centers, ensuring compliance with regulatory requirements for production processes and traceability information, and are stably supplied. All inactivated pathogens undergo infectivity tests on cultured cells and are accompanied by a Certificate of Analysis (COA) to ensure safety and non-infectiousness. Pathogenic microorganisms can be cultured to high concentrations and quantified via TCID50 and qPCR.*Due to frequent inventory changes or additions, please consult our customer service for the availability of in-stock products.