This system is based on a co-expression mechanism using custom plasmids, Taking the novel coronavirus as an example, it combines recombinant green fluorescent protein (rGFP) with the coronavirus 3CL protease. In this system, the rGFP sequence incorporates specific cleavage sites for the 3CL protease.

DIFF Biotech's exclusive DIFF-HPV pseudovirus includes multiple reporter genes such as GFP, RFP, BFP, and Luciferase. It features a high signal-to-noise ratio, strong stability (resistant to 5 freeze-thaw cycles), and requires only cultivation in ordinary 293T cells. It covers the primary types of nine-valent HPV, including types 16, 18, 6, 11, 31, 33, 45, and 52. The pseudovirus itself has a single-cycle infection characteristic, requiring a low biosafety level in the laboratory, making it highly suitable for the PBNA method (pseudovirus-based neutralization assay, PBNA) in HPV neutralization assays.

The viruses of our company originate from the world's largest repository of pathogenic microorganisms and the National Microbial and Virus Strain Preservation Center, with a comprehensive range of virus types and subtypes. The viral strains are authenticated by authoritative microbial strain and virus strain preservation centers, ensuring compliance with regulatory requirements for production processes and traceability information, and are stably supplied. All inactivated pathogens undergo infectivity tests on cultured cells and are accompanied by a Certificate of Analysis (COA) to ensure safety and non-infectiousness. Pathogenic microorganisms can be cultured to high concentrations and quantified via TCID50 and qPCR.*Due to frequent inventory changes or additions, please consult our customer service for the availability of in-stock products.